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- Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521:274-276.
- Bradbury A, Trinklein N, Thie H, Wilkinson I, Tandon A, Anderson S, et al. When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. MAbs. 2018;10(4):539-546.
- Bradbury A, Plückthun A. Reproducibility: Standardize antibodies used in research. Nature. 2015;518(7537):27-29.
- Bradbury A, Sidhu S, Dübel S, McCafferty J. Beyond natural antibodies: the power of in vitro display technologies. Nature Biotechnology. 2011;29:245-254.
- Cosson P, Hartley O. Recombinant antibodies for academia: A practical approach. Chimia (Aarau). 2016;70(12):893-897.
- Dübel S, Stoevesandt O, Taussig MJ, Hust M. Generating recombinant antibodies to the complete human proteome. Trends Biotechnol. 2010;28(7):333-339.
- Echko M, Dozier S. Recombinant antibody technology for the production of antibodies without the use of animals. AltTox.org. 2010.
- Gray A, Sidhu S, Chandrasekera PC, Hendriksen C, Borrebaeck C. Animal-friendly affinity reagents: Replacing the needless in the haystack. Trends Biotechnol. 2016;34(12):960-969.
- Groff K, Allen D, Casey W, Clippinger AJ. Increasing the use of animal-free recombinant antibodies. ALTEX. 2020;37(2):309-311.
- Groff K, Brown J, Clippinger AJ. Modern affinity reagents: Recombinant antibodies and aptamers. Biotechnol Adv. 2015;33(8):1787-1798. (click here for video summary)
- Wenzel EV, Bosnak M, Tierney R, Schubert M, Brown J, Dübel S, Efstratiou A, Sesardic D, Stickings P, Hust M. Human antibodies neutralizing diphtheria toxin in vitro and in vivo. Sci Rep. 2020; 10(571): doi:10.1038/s41598-019-57103-5.
Webinars
- ICCVAM Communities of Practice Webinar 2020: Use of aimal-free affinity reagents
- Bio-Rad: Recombinant antibody webinars
- The making of recombinant anti-idiotypic antibodies for high performance in bioanalytical assays
(hosted by Bio-Rad; 2016) - For information about recombinant antibodies and the scientific drawbacks of animal-derive monoclonal and polyclonal antibodies, see Antibody specificity? What’s the problem and Getting to Recombinant Antibodies that Guarantee Reproducible Research