In Silico / In Vitro Testing Resources

3T analytik manufactures AUREA gTOXXs, an automated screening solution for assessment of the genotoxicity potential of ingredients in pharmaceutical, food, cosmetic, nanoparticle, and medical products. The gTOXXs assay detects DNA damage and repair using automated endpoint measurements and is applicable to three-dimensional tissue models, human organ cells, and cell culture, e.g. the hepatocyte-like HepaRG cell line.

Abich provides in vitro biocompatibility testing for medical devices; in vitro safety and efficacy testing for cosmetics; and in vitro acute toxicity, mutagenesis, carcinogenicity, and ecotoxicity testing for raw materials and ingredients. For cosmetic ingredients, it offers cytotoxicity test for the identification of substances with acute oral toxicity LD50>2000 mg/kg, ocular irritation test (OECD TG 492), in vitro short term exposure test for eye damage (OECD TG 491), in vitro skin irritation (OECD TG 439), and in vitro skin sensitisation (OECD TG 442D). For medical devices, it offers skin irritation testing (ISO 10993-23:2021) and wound healing testing via epidermal cell cultures. It also offers the H295R steroidogenesis assay (OECD TG 456) for medical device and cosmetic ingredients.

Alcyomics, Ltd., offers in vitro human-based pre-clinical drug safety testing. It manufactures Skimune (human skin explants for safety and efficacy testing) and offers immunogenicity services, the Monocyte Activation Test (MAT) for pyrogenicity, efficacy testing of immunomodulators, hypersensitivity testing, potency assessment, cellular and cytokine assays, and safety assessment services.

AllCells, LLC, offers primary tissues and isolated cells collected from a diverse pool of donors.

AlveoliX provides a Lung-on-Chip system and a Barrier-on-Chip system to laboratories for preclinical testing. The Barrier-on-Chip system enables the study of biological barriers such as the lung or gut barrier in order to replicate the complex micro environment of organ barriers taking into account​ the mechanical forces. With the Lung-on-Chip system, healthy, diseased, and personalized in vitro models can test molecule safety, efficacy, and toxicity (drugs, pollutants, cosmetics, etc.) as well as answer fundamental research questions. ​

Attagene, Inc., offers unique screening services for the evaluation of biological activities and prediction of potential toxicities of pharmaceuticals, agricultural and industrial chemicals, cosmetics, and nanomaterials. Using its proprietary pathway profiling platform, the FACTORIAL™, Attagene has developed a suit of bioassays that assess the impact of a compound on activities of over 90 gene regulatory pathways and all 48 human nuclear receptors.

AxoSim produces Nerve-On-A-Chip technology and a three-dimensional brain organoid.

BioScreen Testing Services, Inc., routinely conducts human repeat insult patch tests (HRIPT) and offers a variety of in vitro product assessment services. HRIPT results can be used in place of animal-based skin irritation and sensitisation tests for certain products. BioScreen offers in vitro eye testing (Optisafe in vitro Ocular Irritation Assay, HET-CAM Severe Ocular Irritation and Corrosion Assay, EpiOcular [OECD TG 492], Short Time Exposure [OECD TG 491], and BCOP [OECD TG 437]) and in vitro skin testing (reconstructed human epidermis test methods [OECD TG 439 and 431]).

BioTeSys GmbH provides chemical analysis, clinical trials, and in vitro analysis (including OECD TG 487 for genotoxicity) for sectors such as the food industry, cosmetics, and consumer healthcare.

Celentyx, Ltd., offers analysis of drug action on human immune cells and functional assays to predict potential therapeutic utility in a number of conditions.

Cell Alternativ works with clients to develop in vitro testing strategies for toxicity and product claims for chemicals, cosmetics, pharmaceuticals, and other products. It offers testing for skin irritation (OECD TG 439), skin corrosion (OECD TG 431), and ocular irritation (OECD TG 492) for raw materials and mixtures and testing for cytotoxicity (ISCO 10993-5) and skin irritation (ISO 10993-23) for medical devices and their extracts.

Cellasys GmbH offers system solutions for real-time analysis of living cells, including various lab-on-a-chip technologies to monitor parameters such as cellular viability, pH, and metabolism. Testing services include drug testing, food testing, chemical risk assessment and research on diabetes and nutrition.

CellSystems Biotechnologie Vertrieb GmbH develops and produces primary cell culture systems, stem cell culture systems, and three-dimensional cell culture models.

Central BioHub offers biobanking services and human biospecimens for research and development purposes.

Centrillion Technologies provides sequencing, high-throughput genotyping, and bioinformatics services.

Certara offers computational modeling and simulation services, such as the Simcyp population-based simulator, which is used to model pharmacokinetics and pharmacodynamics in virtual populations and thereby predict drug-drug interactions and pharmacokinetic outcomes in clinical populations, and Model-based Meta-analysis (MBMA), which quantifies clinical trial efficacy and safety information.

chiron develops on-chip biotechnologies with a focus on platforms that apply physiological biomechanical strains to three-dimensional cultures. Its novel technology is ideally suited for musculoskeletal organ-chip models, enabling research into diseases like osteoarthritis and rheumatoid arthritis. chiron collaborates with research institutes and provides customer-service focused research services to the pharmaceutical industry.

ClinWorld provides in vitro pre-clinical research and testing for evaluating toxicity (including dermal, ocular, cytotoxicity, and genotoxicity studies) and efficacy.

CN Bio Innovations, Ltd., provides PhysioMimix organ-on-a-chip systems to identify drug targets, understand drug ADME properties, and assess mechanisms of efficacy and toxicity.

Cultex® Laboratories supplies products for in vitro toxicological analysis of airborne substances, such as gases, particles, volatile compounds, and complex gas mixtures at the air-liquid interface to simulate realistic atmospheric conditions (indoor and outdoor).

Cutest Systems, Ltd., produces customised safety and efficacy dermatological tests for cosmetics, toiletries, over-the-counter preparations, and dermatological products.

Cyprotex provides high-throughput ADME screening and customised ADME assays, in silico pharmacokinetics modelling, in vitro toxicology, endocrine disruption services, personal care and cosmetics industry services, chemical industry services, integrated drug discovery services, and physicochemical profiling services.

Cytox provides biological testing of medical devices, cosmetics, textiles, laquers, and consumer products. Tests include cytotoxicity tests (ISO 10993-5), skin irritation tests (ISO 10993-10, OECD TG 431), epithelial tissue cultures, use of luminescent bacteria (ISO 11348), and photometry/ELISA applications.

Dermal Technology Laboratory, Ltd., conducts percutaneous dermal absorption studies (OECD TG 428) that are needed as part of the safety testing of new and existing chemicals, agrochemicals, and personal care and pharmaceutical products. The company also offers the in vitro epidermis screening test (INVEST) for product selection and formulation optimization.

denovoMATRIX develops, characterizes, and manufactures chemically defined extracellular matrix pre-coated cultureware as well as a screening tool to assess cell microenvironments.

DNTOX offers neurodevelopmental and neuronal testing services using two-dimensional and three-dimensional human cell models and artificial intelligence-based screening with in-house developed software.

Empiriko provides proprietary Biomimiks™ technology that mimics in vivo metabolism, serving as “chemosynthetic livers” for predicting metabolism patterns, pathways, and profiles. This technology can be used to rapidly test and select drug candidates and screen metabolites.

Emulate develops organs-on-chips technology to better understand how different diseases, medicines, chemicals, and foods may affect human health.

Epithelix Sárl manufactures and sells human respiratory tissues for use in in vitro testing, including MucilAir™, a three-dimensional human upper airway epithelia construct; SmallAir™, a three-dimensional human small airway epithelia construct; and Alveolair™, a three-dimensional human alveolar epithelimum construct. The company also offers cryopreserved cells and tissue extracts, and it provides contract testing for various acute and chronic in vitro studies, including inhalational toxicity and drug delivery testing. This company is GIVIMP-certified.

FUJIFILM Cellular Dynamics, Inc. manufactures human induced pluripotent stem cells (iPSCs) and custom iPSCs and iCell® products. The products can be used for drug discovery and screening, testing the safety and efficacy of drug candidates, stem cell banking, and researching cellular therapeutics.

Genetic Resources Core Facility provides equipment and services for use in human genomics studies, including sequencing and genotyping.

Genoskin provides skin model testing kits developed from donated human skin for use in efficacy and toxicity studies. The systems allow for topical or systemic application and subcutaneous or intradermal administration and infusion.

Harmonic Pharma offers in silico services for evaluating chemical efficacy and toxicity assessment. Computational model offerings range from biological profiling to predictive toxicology for pharmaceutical, cosmetics, and chemical sectors.

HemoGenix, Inc., is a contract testing laboratory for the biopharmaceutical industry providing in vitro predictive toxicity testing tools for hemotoxicity, immunotoxicity, hepatotoxicity, neurotoxicity, cardiotoxicity, renal toxicity, skin toxicity, and drug-drug interaction. Assays developed by HemoGenix are available as services and assay kits for in-house use.

HOT Screen focuses on immuno-pharmacological and immuno-toxicological in vitro characterisation of substances under in vivo-like conditions using complex culture systems that combine primary organ cells and tissues with whole blood to approximate interactions with the human immune system. It offers services to pharmaceutical, biotech, cosmetic, chemical, and food supplement companies and medical device manufactures.

In Vitro International is a provider of non-animal testing methods, including the Corrositex method to assess skin corrosion (OECD TG 435) and the ocular in vitro macromolecular test method (OECD TG 496).

INDIGO Biosciences, Inc., provides receptor reporter screening services and kits, gene expression profiling, luminescent cell viability testing, safety pharmacology assays, and custom assay development.

Infinite Biotech, LLC, offers in vitro- and in silico-based toxicity assessment technologies for the prediction of several chronic diseases after exposure to chemicals and particles.

InSphero AG develops three-dimensional microtissues, spheroids, human cryopreserved tumor models, and organ-on-chip platforms for pre-clinical screening and drug safety and efficacy testing, including chronic toxicity testing, cancer research, and disease modelling.

Instem offers a chemoinformatics platform allowing researchers integrated access to chemistry-based data mining, visualisation, and advanced informatics techniques (prediction tools, scaffold generators, etc.). Its prediction technologies allow expert reviews for genetic toxicity, skin sensitisation, carcinogenicity, acute toxicity, reproductive and developmental toxicity, organ toxicity, and environmental toxicity.

Institute for In Vitro Sciences is a nonprofit research and testing laboratory whose offerings include ocular irritation (OECD TG 437, 492, 491, 496, and 437), percutaneous absorption (OECD TG 428), dermal irritation (OECD TG 439), dermal corrosion (OECD TG 431 and 435), phototoxicity (OECD TG 432 and 498), genotoxicity (OECD GD 231, Reconstructed Skin Micronucleus, ToxTracker®), respiratory toxicology, and dermal sensitisation (OECD TG 442C, 442D, and 442E) testing, among other toxicity and efficacy testing assays. The Institute for In Vitro Sciences also has an extensive education and outreach program, including its International Outreach Program.

International Institute for the Advancement of Medicine provides donated organs and tissues for research and education through partnerships with organ procurement organizations, tissue banks, hospitals, and individual donors.

Invitrolize offers three-dimensional alveolar in vitro models allowing to predict the hazard of respiratory sensitisation and irritation. It provides training and support for customers using the models and can provide parallel testing in-house to support confidence in the data.

InvivoSciences, Inc. is a contract research organization offering human engineered tissue-based high content analysis and human micro-heart tissues for drug discovery.

IOMICS Corporation is an analytics company that utilises billions of data points of scientific and clinical data along with automated machine learning, bioinformatics, computational chemistry, and advanced network sciences in drug discovery and disease modeling.

IVTech designs advanced three-dimensional dynamic cell culture systems and custom products for in vitro models and provides workshops and practical training on the use of modular bioreactors and dynamic cell cultures.

J-TEC (Japan Tissue Engineering Co., Ltd.) cultures human tissues such as skin and corneal models for use in in vitro research applications.

Kirkstall, Ltd., produces cell culture chambers that can be connected in a modular fashion using flow of media to create physiologically-relevant conditions for in vitro cell culture. Their chambers come in multiple formats, including air-liquid interface chambers.

Kiyatec, Inc., specialises in providing two and three-dimensional cell-based assays, human ex vivo models, and organoids for prediction of patient response to drugs.

Lhasa Limited, a not-for-profit organisation and educational charity, facilitates collaborative data sharing projects in the pharmaceutical, cosmetics, and chemistry-related industries. Lhasa Limited draws on more than 30 years of experience to create user-friendly, state-of-the-art in silico prediction and database systems for use in metabolism, toxicology, and related sciences.

LifeNetHealth provides in vitro biocompatibility assays for medical devices, cytotoxicity and ADME assays, and a multi-organ platform screening service. It also offers healthy and diseased human tissues and cells.

Marin Biologic Laboratories, Inc., is a contract laboratory that provides in vitro potency assays,  bioassay services for the biotechnology, pharmaceutical, medical device, diagnostic, and agricultural industries.

MAT BioTech B.V. is a biotechnology company with almost 20 years of experience in monocyte activation testing. At global scale, the firm provides pharmaceutical and medical device manufacturers an all-in-one monocyte activation test (MAT) system using peripheral blood mononuclear cells. MAT BioTech also offers outsourced GMP certified MAT services, training, and technical support.

MAT Research is a biotech startup that has developed a monocyte activation test (MAT) kit using human cryopreserved pooled peripheral blood mononuclear cells. In addition to the MAT kit, it provides consulting services and technical support in setting up the MAT.

MatTek Corporation produces human-derived three-dimensional tissue models, including EpiOral, EpiGingival, EpiOcular, EpiAirway, EpiAlveolar, EpiAirway, EpiIntestinal, EpiVaginal, and EpiDerm, among others, for research and testing. The EpiDerm model has been validated for use in the assessment of skin corrosion and irritation (OECD TGs 431 and 439, respectively), and EpiOcular has been validated for assessment of eye damage (OECD TG 492). It also offers toxicology services for skin irritation (OECD TG 439), skin corrosion (OECD TG 431), eye irritation (OECD TG 492), phototoxicity (OECD TG 498), inhalation toxicology, and oral irritation.

McKinney Specialty Labs provides in vitro testing of solids, liquids, and gases, and consulting services. It offers mutagenicity (Ames test [OECD TG 471]), genotoxicity (in vitro micronucleus assay [OECD TG 487]), and cytotoxicity (neutral red uptake assay [OECD TG129]) regulatory toxicology testing following GLP guidelines.

MicroCoat Biotechnology GmbH is a biotechnology company with more than 18 years of experience in the coating of solid phases, kit manufacturing, and diagnostic testing. MicroCoat provides laboratory services, including anti-drug antibodies (ADA) immunogenicity assays, pharmacokinetic assays, custom assay development, and evaluation studies for new diagnostic tests.

Micronit Microfluidics designs, develops, and manufactures custom-made microfluidic components for organ-on-a-chip technology.

MIMETAS, a Netherlands based spin-off company from the University of Leiden, produces human three-dimensional tissue models for use in custom projects and as ready-to-use products.

MultiCASE Inc. provides computational toxicity and metabolite predictions through their CASE Ultra, QSAR Flex, and META Ultra products covering a wide array of toxicity endpoints.

Nelson Laboratories Bozeman LLC. is a testing laboratory that conducts investigative studies for both standard and customised in vitro and clinical studies. It offers testing for topical antimicrobial products, disinfectants, wound care, medical devices, cosmetics, and personal care products. This includes skin corrosion and irritation (OECD TG 431, 437, 439, and 435) and ocular damage (OECD TG 492) testing.

Nortis develops microfluidic chips and a supporting perfusion platform that offer the flexibility to generate a variety of tissue architectures based on cell types and matrix materials selected.

Novoheart develops bioengineered human heart constructs and offers screening and phenotyping services using these technologies.

Organovo designs and creates functional human tissues using its proprietary three-dimensional bioprinting technology for research and drug discovery applications.

Oroxcell offers a broad spectrum of technologies for assessing Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) and efficacy and in vitro toxicity assays to meet regulatory needs.

PeptiMatrix provides access to an animal-free peptide hydrogel platform for three-dimensional cell culture. Designed to compete with animal-derived materials such as Matrigel, PeptiMatrix hydrogels are chemically defined, have limited batch variability, offer better handling properties, and can be customised to mimic a variety of human tissue types.

Pion, Inc., provides products and analytical services for physicochemical characterization (pKa, logP, logD), subcutaneous injection simulation, and to assess drug permeability, absorption and FLUX, solubility, and dissolution.

ProImmune, Ltd., provides in vitro assays to characterise immune responses in drug development.

Propagenix has developed skin and airway tissues and collaborates with pharmaceutical and biotechnology companies that wish to use the technology.

Puracyp research is focused on high-volume screening of new chemical entities to identify potential inducers of P450 enzymes and ABC transporters. Puracyp’s nuclear receptor activation service and products utilise either stably integrated cell lines or transiently transfected cells harbouring P450 enhancer elements in reporter gene constructs and human or animal nuclear receptors, including the Pregnane X Receptor (PXR) and Constitutively Activated Receptor (CAR).

PyroDex uses monocyte activation test technology to test medical devices and therapeutic products for microbial contamination.

QCIF (the Queensland Cyber Infrastructure Foundation) provides bioinformatics services, such as scientific software development, for researchers to analyse and manage large-scale datasets.

Qima Life Sciences provides computational and in vitro research and testing services to the pharmacology, dermatology, cosmetics, and veterinary industries.

RadaHaim has developed an automated, high-throughput protocol for any target cell and/or organ-derived organoid. They offer organ-on-a-panel and body-on-a-chip for research and toxicity and efficacy testing.

ReproCELL Inc., is a contract research organisation offering a range of safety, efficacy, and ADME assays in human fresh tissues. It also provides stem cell products and services and three-dimensional scaffold technology that can be used to culture numerous types of cells.

Research Institutes of Sweden (RISE) offers in vitro toxicity, proliferation, gene expression, protein analysis, and morphology studies in cell lines, in vitro analysis to study interactions between the small intestine and substances or microbiota in EpiIntestinal and Caco2 cells, and simulation software in toxicology.

SABEU manufactures products for use in in vitro research and testing, including filter membranes and standard and custom cell culture inserts. It collaborates with customers on the development of specific plastic molding products and membranes.

ScitoVation develops and implements in vitro and computational approaches to assess PBPK and potential health effects of drugs, food ingredients, chemicals, cosmetics, and other consumer products. This includes assessment of genotoxicity (OECD TG 487 among other assays) and liver, thyroid, and respiratory toxicity testing.

Senzagen offers services to classify skin and respiratory sensitisers using their Genomic Allergen Rapid Detection (GARD) assays. These assays, GARDSkin (OECD TG 442E) and GARDAir, use genomic biomarker signatures for hazard prediction of chemicals and medical devices (for GardSkin). It also offers regulatory toxicity testing for skin irritation (OECD TG 439), skin corrosion (OECD TG 431), skin sensitisation (OECD TG 442C, 442D and 442E), skin absorption (OECD TG 428), eye irritation (OECD TG 492), phototoxicity (OECD TG 432 and TG 498), genotoxicity (OECD TG 471 and TG 487), and pharmacokinetics/ toxicokinetics and ADME.

SiMPLInext offers cell-culture inserts that are made of ceramic, perforated in a periodic pattern, and are 20x thinner than traditional plastic inserts. Leveraging micro fabrication tools, SiMPLInext enables the inclusion of sensors and MEMs in contact with cells for the continuous monitoring of their metabolism, confluence, and health.

Simulab Corporation is a medical technology company offering simulation training tools that replicate human anatomy.

Simulations Plus develops Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) modelling and simulation software, including GastroPlus and ADMET Predictor.

SkinEthic Laboratories produces human-derived three-dimensional tissue models, including skin, corneal, oral, gingival, and vaginal models. SkinEthic’s EpiSkin model has been validated for use in the assessment of skin corrosion and irritation (OECD TGs 431 and 439, respectively).

Stemina offers an assay using either human embryonic stem cells or human induced pluripotent stem cells to predict developmental toxicity based on changes in cellular metabolism following chemical exposure. Stemina also offers an assay to evaluate the cardiotoxicity potential of drug candidates based on changes in human induced pluripotent stem cell-derived cardiomyocyte metabolism and viability.

StemoniX produces the microBrain, an organoid platform consisting of ready-to-use multi-well plates containing functional microtissues engineered from human induced pluripotent stem cells, for use in high-throughput drug screening.

StratiCELL manufactures three-dimensional human skin models and conducts in vitro safety and efficacy testing using monolayer cultures, reconstituted human epidermis, and ex vivo skin explants for dermatology and cosmetic product development.

SynDaver™ Labs manufactures synthetic human tissues and body parts, including anatomy models, organ models, and the SynDaver™ Synthetic Human, that have been validated against the mechanical, thermal, and physicochemical properties of live tissue and can serve as replacements for live animals in medical device studies, clinical training, and surgical simulation. It also has developed canine, feline, and frog models.

Syntivia conducts in vitro tests, including for biological screening and substantiation of cosmetic claims, on active ingredients and formulas for cosmetics.

SynVivo, LLC offers a microchip platform with a physiologically realistic microfluidic environment permitting the real-time study of cellular behavior and interactions, drug discovery and delivery, ADME, and toxicity effects. It also offers three-dimensional tissue and organ-on-chip models for neuroscience, oncology, and inflammation.

Taihoya manufactures a dynamic culture system that simulates a physiologically relevant microenvironment to culture cells.

TissUse produces multi-organ-chips to simulate the activity of multiple human organs in their physiological context for safety evaluation of drugs, cosmetics, foods, and chemicals and for use in pre-clinical human disease modelling.

ToxBank is a warehouse of compound and toxicity data and a reference resource for cells, cell lines, and tissues of relevance for in vitro systemic toxicity research.

Toxys offers in vitro assays to assess genotoxicity, developmental toxicology, and mechanistic toxicity and provide insight on mode of action. The ToxTracker® assay identifies genotoxic compounds, ReproTracker® detects developmental toxicity, and the ToxProfiler™ assay provides a broad toxicity profile of compounds.

Visikol offers a wide range of contract research services, including three-dimensional cell culture assays, tissue imaging and analysis, digital pathology, and custom drug discovery. It also offers Hµrel liver models, which are cell-based tissue constructs composed of primary cells that are metabolically competent.

VITO (Flemish Institute for Technological Research) is a research and contract testing laboratory that maintains Good Laboratory Practices (GLP)-certified facilities for interlaboratory validation studies.

Vitrocell Systems GmbH manufactures exposure systems for in vitro studies of airborne substances, such as gases, complex mixtures, and nanomaterials. Research applications include the study of combustion exhaust, household chemicals, and tobacco smoke, among others. It also produces skin exposure systems for solid, pasteous, liquid or gaseous substances.

VitroScreen offers GLP certified in vitro pre-clinical and regulatory toxicological services for pharmaceuticals, medical devices, chemicals, cosmetics, nanomaterials, food, biocides, and plant protection products. It offers the following toxicity tests: phototoxicity (OECD TG 432), skin corrosion (OECD TG 431), skin irritation (OECD TG 439), eye irritation (OECD TG 492), skin sensitisation (OECD TG 442C, 442D, 442E), and genotoxicity (OECD TG 487). It also offers irritation and cytotoxicity biocompatibility assays (ISO 10993-1[2018] and ISO 10993-23 [2021]) and skin absorption testing (OECD TG 428). Further, Vitroscreen performs standard and customized stainings, immunohistochemistry (IHC) and immunofluorescence (IF) on three-dimensional tissue models, skin explants and spheroids.

XCellR8 is an animal-free, GLP-accredited laboratory that tests the safety and efficacy of raw materials and finished products for cosmetic and chemical companies and ingredient manufacturers around the world. Its animal-product-free adaptations of existing tests for skin sensitisation, skin and eye irritation, and skin corrosion have gained regulatory recognition from the Organisation for Economic Co-operation and Development and European Chemicals Agency. It offers the following toxicity tests: skin corrosion (OECD TG 431), skin irritation (OECD TG 439), eye irritation (OECD TG 492), skin sensitisation (OECD TG 442C, 442D, 442E), and ocular irritection (OECD TG 496).

Xenometrix AG offers in vitro kits to assess mutagenicity, genotoxicity, endocrine disruption, and skin permeation.

Please contact [email protected] to suggest additional in vitro or in silico testing resources.

Disclaimer: Inclusion on this list does not signify an endorsement from PETA Science Consortium International e.V.

Alternative Method Groups

The European Union Reference Laboratory for alternatives to animal testing (EURL ECVAM) validates methods which reduce, refine, or replace the use of animals for safety testing and efficacy/potency testing of chemicals, biologicals, and vaccines.

The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) is an interagency committee composed of representatives from 17 federal regulatory and research agencies that require, use, generate, or disseminate toxicological testing information used to determine the safety or potential adverse health effects of chemicals and products to which workers and consumers may be exposed. The ICCVAM website contains a wealth of information, including resources for developers of replacement, reduction, and refinement methods (see here).

The Japanese Center for the Validation of Alternative Methods (JaCVAM) promotes reduction, refinement, and replacement of animal testing for the evaluation of chemical substance safety in Japan and establishes guidelines for alternative experimental methods through international collaboration.

The Korean Center for the Validation of Alternative Methods (KoCVAM) seeks to review, validate, and institutionalise alternatives to animal testing through cooperation between domestic and foreign organizations, and it promotes alternative test methods developed by Korean organizations globally.

The Brazilian Centre for the Validation of Alternative Methods (BraCVAM) develops, identifies, and validates alternative methods to replace animal testing and is responsible for reviewing proposals from universities, research institutions, and industries that have developed new methods to be considered for adoption in Brazil.