Alternative Approaches for Acute Inhalation Toxicity Testing

Building upon 2015 workshops on inhalation toxicity testing of nanomaterials (here) and acute systemic toxicity testing (here), the PETA Science Consortium International and the US NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) co-hosted a meeting and webinar series to address current practices for acute inhalation toxicity testing. The goal of this work is to develop and implement approaches that reduce and replace acute inhalation testing in mammals for both global regulatory agencies and non-regulatory purposes.

In follow-up to this workshop, the Science Consortium is currently funding testing to show the utility of in vitro approaches to assess the effects of inhaled substances on the respiratory tract. To further this work, the Consortium also donated five VITROCELL® in vitro inhalation exposure devices (here) and funded the development of a three-dimensional human tissue model of the lower respiratory tract (here).


Clippinger AJ, Allen D, Jarabek AM, Corvaro M, Gaça M, Gehen S, Hotchkiss JA, Patlewicz G, Melbourne J, Hinderliter P, Yoon M, Huh D, Lowit A, Buckley B, Bartels M, BéruBé K, Wilson DM, Indans I, Vinken M. Alternative Approaches for Acute Inhalation Toxicity Testing to Address Global Regulatory and Non-Regulatory Data Requirements:  An International Workshop Report. Toxicol In Vitro. 2018;48:53-70.

Clippinger AJ, Allen D, Behrsing H, BéruBé KA, Bolger MB, Casey W, DeLorme M, Gaça M, Gehen SC, Glover K, Hayden P, Hinderliter P, Hotchkiss JA, Iskandar A, Keyser B, Luettich K, Ma-Hock L, Maione A, Makena P, Melbourne J, Milchak L, Ng S, Paini A, Page K, Patlewicz G, Prieto P, Raabe H, Reinke E, Roper C, Rose J, Sharma M, Spoo W, Thorne PA, Wilson DM, Jarabek AM. Pathway-based predictive approaches for non-animal assessment of acute inhalation toxicity. Toxicol In Vitro. 2018; 52:131-145.


Further reading

Detailed information about the 2015 acute systemic toxicity workshop can be found here, and information about the Consortium’s work to develop an in vitro system to assess subchronic inhalation toxicity can be found here.